Sanjin Kulenovic Biochemistry

Services

Specialized Biochemistry Services

Offering comprehensive biochemical expertise to help companies navigate complex scientific challenges and optimize their research and development.

Contact Me

1. High-Performance Liquid Chromatography (HPLC)

HPLC is a powerful analytical technique used to separate, identify, and quantify components in a mixture. It’s essential for analyzing complex biological samples.

Mobile Phase HPLC Pump Microflow Split valve Micro-injection Monolithic column UV Detector with nanocells

Schematic representation of an HPLC system showing the flow path from mobile phase through detection

2. Mass Spectrometry (MS)

Mass spectrometry provides detailed structural and molecular information by measuring the mass-to-charge ratio of ions. It’s vital for proteomics and metabolomics.

Sample Inlet Ion Source (ESI, MALDI, EI) Mass Analyzer (Quadrupole, TOF) Detector Data System Vacuum System

Components and workflow of a mass spectrometry system

3. Gel Electrophoresis

Gel electrophoresis separates macromolecules based on size, charge, and shape. It’s extensively used for DNA, RNA, and protein analysis.

Power Supply 50-250V + − Protein Sample 1 Protein Sample 2

Setup and components of a gel electrophoresis apparatus with sample migration pattern

4. Polymerase Chain Reaction (PCR)

PCR exponentially amplifies DNA sequences through repeated thermal cycling. It’s fundamental for genetic testing, cloning, and sequencing.

0 20 Cycle Number Denaturation 94-98°C Annealing 50-65°C Extension 72°C

PCR thermal cycling process showing denaturation, annealing, and extension steps

5. Enzyme-Linked Immunosorbent Assay (ELISA)

ELISA is an analytical technique that uses antibodies linked to an enzyme to detect the presence of a specific antigen.

Coat with Antigen Step 1 Add Sample with Antibodies Step 2 Add Enzyme-linked Secondary Antibody Step 3 Add Substrate & Measure Color Change Step 4

Step-by-step ELISA procedure showing antibody-antigen interactions and detection

6. Flow Cytometry

Flow cytometry analyzes physical and chemical characteristics of cells or particles as they flow through a laser beam. It’s essential for cell sorting and analysis.

Sample Preparation Flow Cell Sheath Fluid Laser Forward Scatter Detector Side Scatter Detector Fluorescence Detectors Data Analysis System Waste Scatter Plot

Flow cytometry system showing sample flow, laser excitation, and multiple detection pathways

7. Nuclear Magnetic Resonance (NMR) Spectroscopy

NMR spectroscopy analyzes the magnetic properties of atomic nuclei to determine molecular structure, dynamics, and chemical environment.

Superconducting Magnet Sample RF Transmitter RF Receiver Computer Data Analysis NMR Spectrum B₀ Field

Basic components of an NMR spectrometer with superconducting magnet and RF transmitter/receiver system

8. Western Blot

Western blotting identifies specific proteins in a complex mixture through gel electrophoresis followed by transfer to a membrane and antibody detection.

Step 1: SDS-PAGE Step 2: Transfer to Membrane Transfer Cathode (-) Anode (+) Step 3: Blocking Step 4: Antibody Detection Detected Proteins

Western blot workflow showing electrophoresis, membrane transfer, blocking, and antibody detection steps

9. Gas Chromatography (GC)

Gas chromatography separates and analyzes volatile compounds using a carrier gas through a stationary phase column. It’s valuable for analyzing complex mixtures of organic compounds.

Carrier Gas Flow Controller Sample Injector Column Oven Detector Data System Chromatogram Temperature Program

Gas chromatography system showing carrier gas, injector, column, and detector components

10. Protein Purification

Protein purification isolates a specific protein from a complex mixture through a series of fractionation steps based on different physicochemical properties.

Cell Lysis Centrifugation Precipitation (Ammonium Sulfate) Column Chromatography Dialysis & Concentration Purity Analysis SDS-PAGE Western Blot Mass Spec Activity Assay Purified Protein

Protein purification workflow showing various techniques from cell lysis through final analysis

11. Mammalian Cell Culture

Mammalian cell culture involves growing animal cells under controlled conditions outside their natural environment. Critical for biomedical research, drug discovery, and production of therapeutic proteins.

CO₂ Incubator (37°C, 5% CO₂, 95% Humidity) Culture Medium (DMEM + 10% FBS + Antibiotics) 0.2μm Mammalian Cell Culture Workflow 1 Cell Thawing (37°C water bath) 2 Seeding (0.5-1×10⁶ cells/mL) 3 Growth (37°C, 5% CO₂, 48-72 hrs) 4 Passage with Trypsin-EDTA (80-90% confluency) 5 Harvest or Cryopreservation (10% DMSO) Key Parameters pH 7.2-7.4 • 37°C • 5% CO₂ • 95% Humidity Viability >95% • Doubling Time 18-24 hours

Mammalian cell culture system showing culture flask setup and critical workflow steps

12. Plant Cell Culture

Plant cell and tissue culture enables growth of plant cells, tissues, or organs in sterile conditions using nutrient media. Essential for plant propagation, genetic transformation, and secondary metabolite production.

Plant Growth Chamber (22-25°C, 16:8 Light/Dark) Murashige and Skoog (MS) Medium with Sucrose (2-3%) Air Exchange Plant Tissue Culture Workflow 1 Explant Selection (leaf, stem, meristem) 2 Surface Sterilization (70% ethanol, NaOCl) 3 Callus Induction (2,4-D, NAA, BAP hormones) 4 Organogenesis/Embryogenesis (hormone balance) 5 Acclimatization (hardening) and Transfer to Soil Culture Conditions pH 5.6-5.8 • 22-25°C • 16:8 Light/Dark • 1500-3000 lux Light Source

Plant tissue culture system showing growth media and development stages from explant to regeneration

13. Fungal Culture

Fungal culture techniques grow and maintain yeasts or filamentous fungi under controlled laboratory conditions. Essential for studying fungal biology, producing enzymes, and screening antimicrobial compounds.

Fungal Incubator (25-30°C, 70-80% Humidity) Sabouraud Dextrose Agar Fungal Culture Workflow 1 Inoculation (spores or mycelial fragments) 2 Incubation (25-30°C for 3-7 days) 3 Growth Monitoring (colony morphology, color) 4 Subculturing (transfer to fresh media) 5 Spore Harvest or Metabolite Extraction Common Fungal Media • Sabouraud Dextrose Agar (SDA) – pH 5.6 • Potato Dextrose Agar (PDA) – pH 5.6 • Malt Extract Agar (MEA) – pH 5.4 Microscopic View

Fungal culture system showing growth media, hyphal structures, and typical workflow methods

14. Prokaryotic Cell Culture

Bacterial culture is a method of growing bacterial cells under controlled laboratory conditions. Essential for molecular cloning, protein expression, diagnostics, and studying microbial physiology.

Bacterial Incubator (37°C, 150-200 rpm for liquid) LB Agar LB Broth Bacterial Culture Workflow 1 Inoculation (single colony or glycerol stock) 2 Incubation (37°C, 16-18 hours) 3 Growth Monitoring (OD₆₀₀, colony morphology) 4 Induction (IPTG, arabinose) and Harvesting 5 Cell Lysis and Protein/Plasmid Extraction Bacterial Growth Curve OD₆₀₀ Time (hrs) Lag Log Stationary Death E. coli

Bacterial culture systems showing both solid and liquid media cultures with growth monitoring

1 of 10

Watch, Read, Listen